RNA

Part:BBa_K5319680:Design

Designed by: YuFei Xie   Group: iGEM24_ZJU-China   (2024-10-01)


47CAG-2boxB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 316
    Illegal PstI site found at 338
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 260
    Illegal NheI site found at 306
    Illegal SpeI site found at 316
    Illegal PstI site found at 338
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 316
    Illegal PstI site found at 338
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 316
    Illegal PstI site found at 338
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 221
    Illegal BsaI.rc site found at 386


Design Notes

One SpeI restriction site is added between the two box B sequences, allowing one to insert multiple box B sequences in between.


Source

λN peptide-box B RNA hairpin interaction is identified from lambda bacteriophage.

References